Search Result
Results for "
fluorescent substrate
" in MedChemExpress (MCE) Product Catalog:
2
Biochemical Assay Reagents
2
Isotope-Labeled Compounds
Cat. No. |
Product Name |
Target |
Research Areas |
Chemical Structure |
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- HY-P3949
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Fluorescent Dye
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Others
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Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate .
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- HY-P3948
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Fluorescent Dye
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Others
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Fluorescent Substrate for Pro-Specific Proteases is a fluorescent substrate of pro-specific proteases. Fluorescent Substrate for Pro-Specific Proteases can be used to detect the hydrolysis rate and activity of target enzyme .
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-
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- HY-P3947
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Ser/Thr Protease
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Infection
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Fluorescent Substrate for Subtillsin can be used to detect the enzyme activity of subtillsin. Subtilisin is a bacterial serine protease .
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- HY-P3950
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Fluorescent Dye
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Others
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Fluorescent Substrate for Asp-Specific Proteases is a substrate to cleave specifically the Asp-Val bond .
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-
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- HY-P3951
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-
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- HY-129096
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Fluorescent Dye
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Others
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IDT307, an analog of the organic cation MPP+, is a specific fluorescent substrate for DAT (fluorescent substrate APP+) .
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-
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- HY-137834
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Fluorescent Dye
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Others
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Gly-Pro-AMC hydrobromide is a fluorescent dye, it can be used as a specific fluorescent substrate for detecting Dipeptidyl peptidase IV (DPP-IV) activity .
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-
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- HY-P3474
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MMP
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Cancer
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Mca-Pro-Leu-Gly-Leu-Dap-Ala-Arg-NH2 is a fluorescent peptide MMP substrate .
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-
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- HY-137845
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Fluorescent Dye
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Others
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4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer .
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- HY-P3474A
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MMP
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Cancer
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Mca-Pro-Leu-Gly-Leu-Dap-Ala-Arg-NH2 (TFA) is a fluorescent peptide MMP substrate .
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-
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- HY-129887
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Fluorescent Dye
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Others
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Fluorescein diphosphate tetraammonium is a fluorescent dye, it can be used as a fluorogenic substrate and generats fluorescein as a fluorescent product .
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-
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- HY-141575
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Fluorescent Dye
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Others
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C12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays .
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-
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- HY-P4417A
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Fluorescent Dye
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Others
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Ac-IEPD-AMC TFA is a fluorescent substrate used to measure protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC fluoresces under UV light irradiation and can emit fluorescent signals .
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- HY-D1342
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Fluorescent Dye
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Cancer
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Coumberol is a fluorescent substrate of AKR1C3 protein. Coumberol can be used for the research of AKR1C3 .
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-
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- HY-W345102
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-
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- HY-P10142
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Fluorescent Dye
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Others
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Ac-YEVD-AMC is a fluorescent substrate of caspase-1/11. Ac-YEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection when treating with cell lysate .
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- HY-131498
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Fluorescent Dye
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Others
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MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2) and MMP-7. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues .
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-
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- HY-P2065
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Fluorescent Dye
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Others
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Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm) .
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- HY-P3791
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Fluorescent Dye
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Others
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N-Succinyl-Ile-Ile-Trp-AMC is a peptide-based fluorescent substrate or probe for carboxypeptidase Y (CPY) .
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- HY-P4400
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Fluorescent Dye
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Others
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Z-VDVAD-AFC is a fluorogenic substrate. Z-VDVAD-AFC is used to measure the activity of cysteine protease 3 (Caspase-3). Z-VDVAD-AFC undergoes hydrolysis to release 7-amino-4-trifluoromethylcoumarin (AFC). AFC is fluorescent under ultraviolet light and can emit fluorescent signals .
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- HY-141577
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Biochemical Assay Reagents
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Others
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C12-NBD Sphinganine is a fluorescent ceramidase substrate. C12-NBD Sphinganine can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources .
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- HY-D1678
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Fluorescent Dye
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Others
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5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .
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- HY-P10144
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Fluorescent Dye
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Others
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Ac-Orn-Phe-Arg-AMC is a potent fluorescent substrate of cd–Tryptase and β 1-Tryptase. Ac-Orn-Phe-Arg-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection .
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- HY-113862
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Fluorescent Dye
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Others
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PHOME is a fluorogenic substrate for sEH. sEH can hydrolyze the epoxy ring in the PHOME substrate. PHOME can be used for fluorescent epoxide hydrolase assay (extracted from patent CN113402447A) .
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- HY-148073
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- HY-157744
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Others
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Coumarin–quinone conjugate is a fluorescent substrate for NADH:ubiquinone oxidoreductases which consists of a coumarin fluorophore and a ubiquinone analog. Coumarin–quinone conjugate can be used to measure the kinetic parameters of AIFM2/FSP1 for researches such as ferroptosis .
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- HY-P2615
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Fluorescent Dye
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Cancer
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Ac-VDVAD-AFC is a caspase-specific fluorescent substrate. Ac-VDVAD-AFC can measure caspase-3-like activity and caspase-2 activity and can be used for the research of tumor and cancer .
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- HY-W030319
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Fluorescent Dye
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Others
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Methyl 2-(7-hydroxy-2-oxo-2H-chromen-4-yl)acetate, a natural coumarins derivative, is exploited for the synthesis of the switchable fluorescent substrates to be used in bacterial enzyme detection .
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- HY-118155
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Fluorescent Dye
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Others
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9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H +/solute antiport system .
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- HY-123633
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Glucosidase
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Others
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4-Methylumbelliferyl β-D-Glucopyranoside, a β-D-glucoside, is a fluorogenic substrate for β-glucosidase, utilizes to assay β-glucosidase activity . 4-Methylumbelliferyl β-D-Glucopyranoside releases the highly fluorescent 4-methylumbelliferyl (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively .
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- HY-P10174
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- HY-D1685
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Fluorescent Dye
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Others
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BZiPAR is a fluorescent probe. BZiPAR also is a substrate of trypsin that becomes fluorescent after cleavage by the protease .
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- HY-P5990
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PSMA
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Cancer
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Prostate Specific Antigen Substrate is a prostate specific antigen (PSA) fluorescent substrate. Prostate Specific Antigen Substrate can be used for detect enzymatic activity of PSA .
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- HY-P4931
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Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2
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MMP
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Cancer
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Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1 . (Ex/Em=325 nm/400 nm)
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- HY-P4401
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Caspase
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Others
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Z-VEID-AFC is a fluorescent substrate for caspase-6 .
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- HY-D1740
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DHTM Ros
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Fluorescent Dye
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Others
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Dihydrotetramethylrosamine (DHTM Ros) is a fluorogenic substrate for peroxidase that oxidizes to fluorescent tetramethylrosamine chloride.
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- HY-W019823
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- HY-P4344
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MetAP
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Others
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Met-Gly-Pro-AMC is a fluorescent peptide substrate of MetAP2 .
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- HY-P1853
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-
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- HY-119395B
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- HY-119395A
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- HY-136143
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Others
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Others
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(BHQ-3)-OSu hexafluorophosphate is used for fluorescent labeling of protease substrates .
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- HY-D2205
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Cy7 TSA
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Fluorescent Dye
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Others
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Cy7 tyramide, a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
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- HY-128919
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HDAC
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Others
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Ac-Lys-AMC (Hexanamide), also termed MAL, is a fluorescent substrate for histone deacetylase HDACs .
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- HY-D1529
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Fluorescent Dye
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Others
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4-Methylumbelliferyl phosphate (2-amino-2-methyl-1,3-propanediol) is a fluorescent substrate, can be used as substrate buffer of enzyme assay .
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- HY-D0149
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Cytochrome P450
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Others
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4-(Trifluoromethyl)umbelliferone is fluorescent probe substrate for rat hepatic cytochrome P450 enzymes .
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- HY-P1093
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Caspase
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Others
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Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase .
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- HY-D1698
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Fluorescent Dye
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Others
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PED6 is a PLA2 substrate fluorescent probe (EX/EM=488/530 nm) that is a BODIFY-labeled fluorescent phosphatidylethanolamine analogue. PED6 can be used for detection of phospholipase A2 .
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- HY-D1582
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Fluorescent Dye
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Others
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C12 NBD Phytoceramide, a ceramide analogue, is a fluorescent substrate, can be used to detect ceramidase activity .
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- HY-137855
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Biochemical Assay Reagents
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Others
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4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
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- HY-137592
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ε-NAD
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Fluorescent Dye
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Others
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Nicotinamide 1,N6-ethenoadenine dinucleotide (ε-NAD) , a fluorescent analogue of NAD, is able to serve as a substrate for the bacterial toxincatalyzed G-ADP ribosylation of signal-transducing G-proteins. Nicotinamide 1,N6-ethenoadenine dinucleotide can be used as a fluorescent substrate for the studies of the ADP ribosylation reaction .
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- HY-131007
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Adrenergic Receptor
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Metabolic Disease
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FFN270 hydrochloride, a fluorescent tracer of norepinephrine, is a fluorescent substrate of the norepinephrine and vesicular monoamine transporters. FFN270 hydrochloride exhibits two resolved absorption/excitation maxima depending on solvent pH (FFN270 ex: 320 nm or 365 nm, em: 475 nm) and can function as ratiometric fluorescent pH-sensors .
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- HY-D0091
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Fluorescent Dye
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Others
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7-Ethoxy-4-trifluoromethylcoumarin is a fluorescent substrate of various enzymes of the cytochrome P450 superfamily
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- HY-136247
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Tyramide-Cy5
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DNA Stain
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Others
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Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
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- HY-D1699
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Fluorescent Dye
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Others
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PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity .
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- HY-P3722
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Fluorescent Dye
MMP
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Others
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Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE; ADAM17), ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis .
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- HY-P3722A
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Fluorescent Dye
MMP
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Others
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Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE); ADAM17, ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis .
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- HY-136248
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Tyramide-Cy3
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DNA Stain
Fluorescent Dye
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Others
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Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
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- HY-P1883
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Fluorescent Dye
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Infection
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Bacterial Sortase Substrate III, Abz/DNP is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.
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- HY-P1883A
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Fluorescent Dye
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Infection
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Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.
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- HY-P10073
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Fluorescent Dye
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Others
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Calpain-1 substrate, fluorogenic serves as a sensitive and specific substrate for calpain-1 that cleaves Tyr-Gly bond and results in enhanced fluorescence .(Ex/Em = 490 nm/518 nm)
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- HY-P2176
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Z-Leu-Arg-Gly-Gly-AMC
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Biochemical Assay Reagents
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Others
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Z-LRGG-AMC (Z-Leu-Arg-Gly-Gly-AMC) is a fluorescent substrate that hydrolyzes and releases free fluorescent 7-amino-4-methylcoumarin (AMC). Detection wavelength of AMC: Ex/Em=360/460 nm .
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- HY-D0055
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Cytochrome P450
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Others
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3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage
Target: Cytochrome P450
3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.
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- HY-136247A
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DNA Stain
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Others
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Cyanine 5 Tyramide (Tyramide-Cy5) methyl indole is a red fluorescent dye. Cyanine 5 Tyramide is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids . Storage: protect from light.
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- HY-136248A
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Fluorescent Dye
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Others
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Cyanine 3 Tyramide methyl indole is a derivative of Cyanine 3 Tyramide (HY-136248). Cyanine 3 Tyramide is an orange fluorescent dye, and is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
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- HY-118135
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4MU-α-Gal
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Fluorescent Dye
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Others
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4-Methylumbelliferyl-α-D-galactopyranoside (4MU-α-Gal), a substrate for α-galactosidase A (GLA), is a blue pro-fluorogenic substrate. 4-Methylumbelliferyl-α-D-galactopyranoside forms two products, galactose and fluorescent 4MU, upon cleavage by GLA .
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- HY-D1532
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Parasite
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Others
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DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye, and can be applied in a fluorogenic substrate for an aspartyl proteinase from human malaria parasite .
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- HY-D1609
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Fluorescent Dye
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Others
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BODIPY FL Verapamil hydrochloride is a fluorescent dye, it can be used as a molecular probe to label tissues and cells. BODIPY FL Verapamil hydrochloride is a substrate for both ABCB1 and ABCC1 .
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- HY-P4217
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Cathepsin
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Others
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Z-Gly-Pro-Arg-AMC hydrochloride a fluorescent trypsin and cathepsin K substrate. Z-Gly-Pro-Arg-AMC hydrochloride can be used to determine trypsin and cathepsin K activity .
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- HY-D1577
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Fluorescent Dye
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Cancer
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C6 NBD Lactosylceramide is a derivative of lactosylceramides, and can be used in lactosylceramide synthase assay as a fluorescent acceptor substrate. C6 NBD Lactosylceramide can also be used in cancer research .
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- HY-W027544
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Fluorescent Dye
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Others
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MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .
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- HY-121941
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Fluorescent Dye
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Others
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Epoxy Fluor 7 is a sensitive fluorescent substrate for soluble epoxide hydrolase (sEH) that can be used for human and mouse enzymes. Epoxy Fluor 7 is hydrolyzed to yield fluorescence used for monitoring the activity of sEH.
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- HY-110210
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BODIPY FL,SE
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Fluorescent Dye
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Others
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BODIPY-FL NHS ester (BODIPY FL, SE) is an amine-reactive fluorescent probe. The maximum excitation/emission values are 502/511 nm. BODIPY-FL NHS ester can be used to synthesize protease substrates .
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- HY-131006
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Monoamine Transporter
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Neurological Disease
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FFN200 dihydrochloride, a fluorescent substrate of VMAT2, selectively trace monoamine exocytosis in both neuronal cell culture and brain tissue. The fluorescence excitation and emission maxima of FFN200 are determined to be 352 and 451 nm, respectively .
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- HY-D1523
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Cathepsin
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Others
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Z-Arg-Arg-4MβNA triacetate is a cathepsin B-specific substrate and can produce fluorescent end product 4MβNA (λex = 355 nm, λem = 430 nm) .
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- HY-139101
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GpppA
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DNA/RNA Synthesis
Endogenous Metabolite
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Others
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Guanosine 5'-triphosphate-5'-adenosine (GpppA), a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine is a fluorescent substrate analog .
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- HY-137928
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Fluorescent Dye
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Others
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H-Ala-4MβNA hydrochloride is a specific fluorogenic substrate for aminopeptidase M .
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- HY-P3475
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Fluorescent Dye
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Others
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Mca-VDQMDGWK-(Dnp)-NH2 is a fluorogenic substrate for caspase-3 and can be used to quantify caspase-3 activity .
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- HY-W277900
-
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MMP
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Inflammation/Immunology
Cancer
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2-Methoxy-2,4-diphenylfuran-3-one is a fluorescent compound which can be used to label gelatin as a substrate for detection of the gelatin degrading MMP-2 and MMP-9 by zymography .
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- HY-P3749
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Fluorescent Dye
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Others
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Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
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- HY-139101A
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GpppA triammonium
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DNA/RNA Synthesis
Endogenous Metabolite
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Others
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Guanosine 5'-triphosphate-5'-adenosine (GpppA) triammonium, a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine triammonium is a fluorescent substrate analog .
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- HY-D1670
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- HY-D1341
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Fluorescent Dye
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Cancer
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Coumberone is a metabolic fluorogenic probe, and isoform-selective substrate for all AKR1C isoforms. Coumberone can be reduced by all four members of the AKR1C family to its fluorescent alcohol coumberol. Coumberone can be used for the research of AKR1C .
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- HY-P3110
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Fluorescent Dye
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Others
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Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity .
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- HY-149170
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5-HT Receptor
Monoamine Transporter
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Neurological Disease
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FFN246 is a fluorescent, dual substrate of serotonin transporter (SERT) probe and vesicular monoamine transporter 2 (VMAT2) with excitation and emission spectra 392/427 nm. FFN246 can be used for labeling serotonergic neurons in mouse brain tissue through SERT-dependent accumulation .
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- HY-P10172
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- HY-W267897
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7-BFC
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Cytochrome P450
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Others
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7-Benzyloxy-4-(trifluoromethyl)coumarin (7-BFC) is a coumarin fluorescent substrate. 7-Benzyloxy-4-(trifluoromethyl)coumarin is a substrate for cDNA-expressed CYP1A2 and CYP3A4 and is metabolized to 7-hydroxy-4-trifluoromethylcoumarin (HFC). 7-Benzyloxy-4-(trifluoromethyl)coumarin is used for rapid CYP isoform metabolism and inhibition screening studies .
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- HY-D1584
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Phospholipase
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Others
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C12 NBD sphingomyelin is an active derivative of sphingomyelin (HY-113498) that is tagged with fluorescent C12 nitrobenzoxadiazole (C12 NBD). C12 NBD sphingomyelin can be used as a sphingomyelinase substrate for studying the metabolism and transport of sphingomyelins (Ex=470 nm, Em=525 nm) .
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- HY-D1575
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Fluorescent Dye
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Others
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C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525) .
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- HY-126839
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5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
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Fluorescent Dye
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Others
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C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells .
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- HY-116141
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7-HCA; Umbelliferyl Arachidonate; 7-HC-arachidonate
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Phospholipase
MAGL
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Others
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7-Hydroxycoumarinyl arachidonate (7-HCA) is a fluorogenic substrate of cytosolic phospholipase A2 (PLA2). 7-Hydroxycoumarinyl arachidonate is also a fluorogenic substrate for monoacylglycerol lipase (MAGL). MAGL protein catalyzes the hydrolysis of 7-Hydroxycoumarinyl arachidonat to generate Arachidonic acid (AA) and the highly fluorescent 7-hydroxyl coumarin (7-HC; HY-N0573). Release of 7-HC can be measured using a fluorometer .
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- HY-P1003
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Fluorescent Dye
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Others
|
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .
|
-
- HY-D1739
-
|
Fluorescent Dye
|
Others
|
DDAO phosphate diammonium is a fluorescent phosphatase substrate. DDAO phosphate diammonium has tunable excitation wavelength (600-650nm) and long emission wavelength (λem=656nm). DDAO phosphate diammonium can be used to detect the activity of different enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin and esterase.
|
-
- HY-P4593
-
|
Thrombin
|
Others
|
Z-Gly-Pro-Arg-4MβNA is the cleavage of the substrate of thrombin to release free 4-methoxy-2-naphthylamine (4MβNA). Free 4MβNA can be captured by 5-nitrosalicylaldehyde to produce an insoluble yellow fluorescent and marks the site of thrombin activity .
|
-
- HY-P4899
-
|
Beta-secretase
|
Neurological Disease
|
RE (EDANS) EVNLDAEFK (DABCYL) R is an EDANS and DABCYL double-labeled peptide,serves as a fluorescent substrate for BACE1(Em=360nm,Ex=528nm). RE (EDANS) EVNLDAEFK (DABCYL) R can be used for BACE1 activity measurement and the enzyme activity level is directly proportional to the fluorescence reaction .
|
-
- HY-110334
-
|
Others
|
Others
|
FFN 206 dihydrochloride, a fluorescent probe, is used as an excellent Vesicular Monoamine Transporter 2 (VMAT2) substrate with an apparent Km of 1.16 μM. FFN 206 dihydrochloride is capable of detecting VMAT2 activity in intact cells using fluorescence microscopy, with subcellular localization to VMAT2-expressing acidic compartments without apparent labeling of other organelles .
|
-
- HY-P4920
-
|
Fluorescent Dye
|
Others
|
Mca-SEVNLDAEFK(Dnp)-NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1 .
|
-
- HY-W027544S
-
7-Methoxy-d3-2-oxo-2H-1-benzopyran-4-acetic acid; 7-Methoxy-d3-2H-chromen-2-one-4-acetic acid
|
Isotope-Labeled Compounds
|
Others
|
(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d3 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3].
|
-
- HY-120659
-
|
Fluorescent Dye
|
Others
|
Gly-Gly-AMC is a fluorogenic peptide substrate. Gly-Gly-AMC can be used to assess bacterial protease activity of P. aeruginosa and S. aureus .
|
-
- HY-P4919
-
|
Beta-secretase
|
Others
|
Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
|
-
- HY-133535
-
PA-JF646-NHS
|
Fluorescent Dye
|
Others
|
PA Janelia Fluor® 646, SE (PA-JF646-NHS), a photoactivatable fluorescent dye, is an NHS ester for coupling to primary amine groups. PA-JF646-NHS is non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g.HaloTag ® and SNAP-tag ®. PA-JF646-NHS is used for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. (λExcitation/emission~650/664 nm). Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
|
-
- HY-D1632
-
|
Fluorescent Dye
|
Others
|
4-MU-α-GlcNS sodium is a fluorogenic substrate of heparin sulphamidase, is desulfurized into 4-MU-α-GlcNH2. 4-MU-α-GlcNH2 can liberate 4-methylumbelliferone (4-MU, fluorescent product) via α-glucosaminidase catalysis, with the emission wavelength maxima of 445-454 nm. 4-MU-α-GlcNS sodium can be used to heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders researches .
|
-
- HY-160250
-
|
UGT
|
Metabolic Disease
|
UGT1A1-IN-1 (compound 2) is a non-competitive inhibitor of UGT1A1, which can inhibit the 1-O-glucuronidation process mediated by UGT1A1 with a Ki value of 5.02 μM. UGT1A1-IN-1 (compound 2) can bind on UGT1A1 at the same ligand-binding site as bilirubin (HY-N0323). UGT1A1-IN-1 can serve as a ‘turn-on’ fluorescent probe substrate for UGT1A1 .
|
-
- HY-131131
-
|
Fluorescent Dye
|
Others
|
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell .
|
-
- HY-W027544S1
-
5-(Dimethyl-d6-amino)-N-(prop-2-ynyl)naphthalene-1-sulfonamide
|
Isotope-Labeled Compounds
|
Others
|
(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
|
-
- HY-W354821
-
4-MUD
|
Fluorescent Dye
|
Metabolic Disease
|
4-Methyl-2-oxo-2H-1-benzopyran-7-yl decanoate is a fluorogenic substrate used to follow the hydrolytic activity of carboxylesterases .
|
-
- HY-W357142
-
-
Cat. No. |
Product Name |
Type |
-
- HY-129096
-
|
Chromogenic Substrates
|
IDT307, an analog of the organic cation MPP+, is a specific fluorescent substrate for DAT (fluorescent substrate APP+) .
|
-
- HY-137845
-
|
Fluorescent Dyes/Probes
|
4-Methylumbelliferyl β-D-galactopyranoside is a fluorescent substrate for β-galactosidase which, when cleaved, produces a water-soluble blue fluorescent coumarin fluorophore that can be detected using a fluoroenzymeter or fluorometer .
|
-
- HY-P3474
-
-
- HY-141575
-
|
Fluorescent Dyes/Probes
|
C12-NBD-ceramide is a fluorescent analogue of ceramide, it can be used as a substrate in ceramidase assays .
|
-
- HY-W345102
-
|
Chromogenic Substrates
|
4-Methylumbelliferyl β-cellotrioside, a chromogenic substrate for β-glycosidases, is a cellulose fluorescent derivative .
|
-
- HY-131498
-
|
Fluorescent Dyes/Probes
|
MOCAc-PLGL(Dpa)AR is a positively charged fluorescent substrate for matrix metalloproteinase-2 (MMP-2) and MMP-7. MOCAc-PLGL(Dpa)AR is a substrate of matrilysin, can be cleaved at the peptide bond between the glycine and leucine residues .
|
-
- HY-P2065
-
|
Fluorescent Dyes/Probes
|
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm) .
|
-
- HY-P3791
-
|
Fluorescent Dyes/Probes
|
N-Succinyl-Ile-Ile-Trp-AMC is a peptide-based fluorescent substrate or probe for carboxypeptidase Y (CPY) .
|
-
- HY-141577
-
|
Dyes
|
C12-NBD Sphinganine is a fluorescent ceramidase substrate. C12-NBD Sphinganine can be used for the measurement of alkaline and neutral ceramidase activity from a variety of sources .
|
-
- HY-D1678
-
|
Fluorescent Dyes/Probes
|
5(6)-Carboxynaphthofluorescein diacetate is a fluorescent substrate for esterase assays that can be cleaved by intracellular esterases, producing red fluorescence to measure enzyme activity (Ex = 590 nm; Em = 645 nm) .
|
-
- HY-113862
-
|
Dyes
|
PHOME is a fluorogenic substrate for sEH. sEH can hydrolyze the epoxy ring in the PHOME substrate. PHOME can be used for fluorescent epoxide hydrolase assay (extracted from patent CN113402447A) .
|
-
- HY-148073
-
-
- HY-118155
-
|
Fluorescent Dyes/Probes
|
9-Amino-6-chloro-2-methoxyacridine is a pH sensitive fluorescent probe. 9-Amino-6-chloro-2-methoxyacridine has been frequently used to measure changes in vacuolar pH when a specific substrate crosses the tonoplast through a putative H +/solute antiport system .
|
-
- HY-D1740
-
DHTM Ros
|
Fluorescent Dyes/Probes
|
Dihydrotetramethylrosamine (DHTM Ros) is a fluorogenic substrate for peroxidase that oxidizes to fluorescent tetramethylrosamine chloride.
|
-
- HY-W019823
-
-
- HY-D2205
-
Cy7 TSA
|
Fluorescent Dyes/Probes
|
Cy7 tyramide, a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids.
|
-
- HY-D1529
-
|
Fluorescent Dyes/Probes
|
4-Methylumbelliferyl phosphate (2-amino-2-methyl-1,3-propanediol) is a fluorescent substrate, can be used as substrate buffer of enzyme assay .
|
-
- HY-D0149
-
|
Fluorescent Dyes/Probes
|
4-(Trifluoromethyl)umbelliferone is fluorescent probe substrate for rat hepatic cytochrome P450 enzymes .
|
-
- HY-P1093
-
|
Chromogenic Substrates
|
Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase .
|
-
- HY-D1698
-
|
Fluorescent Dyes/Probes
|
PED6 is a PLA2 substrate fluorescent probe (EX/EM=488/530 nm) that is a BODIFY-labeled fluorescent phosphatidylethanolamine analogue. PED6 can be used for detection of phospholipase A2 .
|
-
- HY-D1582
-
|
Chromogenic Substrates
|
C12 NBD Phytoceramide, a ceramide analogue, is a fluorescent substrate, can be used to detect ceramidase activity .
|
-
- HY-D0091
-
|
Fluorescent Dyes/Probes
|
7-Ethoxy-4-trifluoromethylcoumarin is a fluorescent substrate of various enzymes of the cytochrome P450 superfamily
|
-
- HY-136247
-
Tyramide-Cy5
|
Fluorescent Dyes/Probes
|
Cyanine 5 Tyramide (Tyramide-Cy5), a red fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
|
-
- HY-D1699
-
|
Fluorescent Dyes/Probes
|
PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity .
|
-
- HY-136248
-
Tyramide-Cy3
|
Fluorescent Dyes/Probes
|
Cyanine 3 Tyramide (Tyramide-Cy3), an orange fluorescent dye, is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
|
-
- HY-D0055
-
|
Chromogenic Substrates
|
3-Cyano-7-ethoxycoumarin is a fluorogenic cytochrome P-450 substrate that generates blue fluorescent product upon enzyme cleavage
Target: Cytochrome P450
3-Cyano-7-ethoxycoumarin is a fluorescent probe useful in microsomal dealkylase studies.
|
-
- HY-136248A
-
|
Fluorescent Dyes/Probes
|
Cyanine 3 Tyramide methyl indole is a derivative of Cyanine 3 Tyramide (HY-136248). Cyanine 3 Tyramide is an orange fluorescent dye, and is utilized as reporter fluorescent substrate for horseradish peroxidase (HRP)-catalyzed deposition that is signal amplification technique in immunoassay and in situ hybridization of nucleic acids .
|
-
- HY-D1532
-
|
Fluorescent Dyes/Probes
|
DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye, and can be applied in a fluorogenic substrate for an aspartyl proteinase from human malaria parasite .
|
-
- HY-D1609
-
|
Dyes
|
BODIPY FL Verapamil hydrochloride is a fluorescent dye, it can be used as a molecular probe to label tissues and cells. BODIPY FL Verapamil hydrochloride is a substrate for both ABCB1 and ABCC1 .
|
-
- HY-D1577
-
|
Fluorescent Dyes/Probes
|
C6 NBD Lactosylceramide is a derivative of lactosylceramides, and can be used in lactosylceramide synthase assay as a fluorescent acceptor substrate. C6 NBD Lactosylceramide can also be used in cancer research .
|
-
- HY-W027544
-
|
Fluorescent Dyes/Probes
|
MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities .
|
-
- HY-121941
-
|
Fluorescent Dyes/Probes
|
Epoxy Fluor 7 is a sensitive fluorescent substrate for soluble epoxide hydrolase (sEH) that can be used for human and mouse enzymes. Epoxy Fluor 7 is hydrolyzed to yield fluorescence used for monitoring the activity of sEH.
|
-
- HY-110210
-
BODIPY FL,SE
|
Fluorescent Dyes/Probes
|
BODIPY-FL NHS ester (BODIPY FL, SE) is an amine-reactive fluorescent probe. The maximum excitation/emission values are 502/511 nm. BODIPY-FL NHS ester can be used to synthesize protease substrates .
|
-
- HY-D1523
-
|
Fluorescent Dyes/Probes
|
Z-Arg-Arg-4MβNA triacetate is a cathepsin B-specific substrate and can produce fluorescent end product 4MβNA (λex = 355 nm, λem = 430 nm) .
|
-
- HY-137928
-
-
- HY-P3475
-
|
Fluorescent Dyes/Probes
|
Mca-VDQMDGWK-(Dnp)-NH2 is a fluorogenic substrate for caspase-3 and can be used to quantify caspase-3 activity .
|
-
- HY-W277900
-
|
Fluorescent Dyes/Probes
|
2-Methoxy-2,4-diphenylfuran-3-one is a fluorescent compound which can be used to label gelatin as a substrate for detection of the gelatin degrading MMP-2 and MMP-9 by zymography .
|
-
- HY-P3749
-
|
Fluorescent Dyes/Probes
|
Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
|
-
- HY-D1670
-
-
- HY-P3110
-
|
Dyes
|
Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity .
|
-
- HY-D1584
-
|
Fluorescent Dyes/Probes
|
C12 NBD sphingomyelin is an active derivative of sphingomyelin (HY-113498) that is tagged with fluorescent C12 nitrobenzoxadiazole (C12 NBD). C12 NBD sphingomyelin can be used as a sphingomyelinase substrate for studying the metabolism and transport of sphingomyelins (Ex=470 nm, Em=525 nm) .
|
-
- HY-D1575
-
|
Dyes
|
C6 NBD galactosylceramide is an active derivative of galactosylceramide that is tagged with fluorescent C6 nitrobenzoxadiazole (C6 NBD). C6 NBD galactosylceramide can be used as a substrate for neutral β-glycosylceramidase (GCase) to study intracellular localization and metabolism of galactosylceramide (Ex=nm, Em=525) .
|
-
- HY-126839
-
5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside
|
Fluorescent Dyes/Probes
|
C12FDG (5-Dodecanoylaminofluorescein di-β-D-Galactopyranoside) is a lipophilic green fluorescent substrate for β-galactosidase detection. C12-FDG is more sensitive than FDG (HY-101895) for beta-galactosidase activity determinations in animal cells .
|
-
- HY-P1003
-
|
Chromogenic Substrates
|
Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .
|
-
- HY-D1739
-
|
Fluorescent Dyes/Probes
|
DDAO phosphate diammonium is a fluorescent phosphatase substrate. DDAO phosphate diammonium has tunable excitation wavelength (600-650nm) and long emission wavelength (λem=656nm). DDAO phosphate diammonium can be used to detect the activity of different enzymes such as β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin and esterase.
|
-
- HY-133535
-
PA-JF646-NHS
|
Dyes
|
PA Janelia Fluor® 646, SE (PA-JF646-NHS), a photoactivatable fluorescent dye, is an NHS ester for coupling to primary amine groups. PA-JF646-NHS is non-fluorescent until activated at 365 nm. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g.HaloTag ® and SNAP-tag ®. PA-JF646-NHS is used for single molecule tracking and super resolution microscopy in live cells, specifically live cell sptPALM and fixed cell PALM. (λExcitation/emission~650/664 nm). Janelia Fluor® products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.
|
-
- HY-D1632
-
|
Fluorescent Dyes/Probes
|
4-MU-α-GlcNS sodium is a fluorogenic substrate of heparin sulphamidase, is desulfurized into 4-MU-α-GlcNH2. 4-MU-α-GlcNH2 can liberate 4-methylumbelliferone (4-MU, fluorescent product) via α-glucosaminidase catalysis, with the emission wavelength maxima of 445-454 nm. 4-MU-α-GlcNS sodium can be used to heparin sulphamidase deficiencies associated with Mucopolisaccaridosis IIIA and other lysosomal disorders researches .
|
-
- HY-131131
-
|
Fluorescent Dyes/Probes
|
5-CFDA-AM is a cell-permeable esterase substrate that can be used as an active probe to measure enzyme activity and cell membrane integrity. 5-CFDA-AM is electroneutral and can enter the cell at a lower concentration than CFDA, where it is hydrolysed by intracellular esterases to produce carboxyfluorescein. Carboxyfluorescein contains an additional negative charge and can be better retained in the cell .
|
-
- HY-W354821
-
-
- HY-W357142
-
Cat. No. |
Product Name |
Type |
-
- HY-137855
-
|
Biochemical Assay Reagents
|
4-Methylumbelliferyl sulfate (potassium), a fluorescent substrate, is commonly used to detect sulfatase activity in biochemical and biomedical research. It consists of a sulfate group attached to a fluorescent molecule, which can be cleaved by sulfatase enzymes. Upon cleavage, 4-Methylumbelliferyl sulfate releases a highly fluorescent product that can be detected using fluorescence microscopy or spectroscopy. The use of 4-Methylumbelliferyl sulfate as a substrate for sulfatase enzymes allows accurate detection and quantification of these enzymes in a variety of biological samples.
|
-
- HY-139101
-
GpppA
|
Gene Sequencing and Synthesis
|
Guanosine 5'-triphosphate-5'-adenosine (GpppA), a 5′ cap analog, can be used for RNA synthesis in vitro. Guanosine 5'-triphosphate-5'-adenosine is a fluorescent substrate analog .
|
Cat. No. |
Product Name |
Target |
Research Area |
-
- HY-P3949
-
|
Fluorescent Dye
|
Others
|
Fluorescent Substrate for Glu-Specific Proteases is a V8 protease-Specific chromogenic substrate .
|
-
- HY-P3948
-
|
Fluorescent Dye
|
Others
|
Fluorescent Substrate for Pro-Specific Proteases is a fluorescent substrate of pro-specific proteases. Fluorescent Substrate for Pro-Specific Proteases can be used to detect the hydrolysis rate and activity of target enzyme .
|
-
- HY-P3947
-
|
Ser/Thr Protease
|
Infection
|
Fluorescent Substrate for Subtillsin can be used to detect the enzyme activity of subtillsin. Subtilisin is a bacterial serine protease .
|
-
- HY-P3950
-
|
Fluorescent Dye
|
Others
|
Fluorescent Substrate for Asp-Specific Proteases is a substrate to cleave specifically the Asp-Val bond .
|
-
- HY-P3951
-
-
- HY-P3474
-
|
MMP
|
Cancer
|
Mca-Pro-Leu-Gly-Leu-Dap-Ala-Arg-NH2 is a fluorescent peptide MMP substrate .
|
-
- HY-P3474A
-
|
MMP
|
Cancer
|
Mca-Pro-Leu-Gly-Leu-Dap-Ala-Arg-NH2 (TFA) is a fluorescent peptide MMP substrate .
|
-
- HY-P4465
-
|
Peptides
|
Others
|
Gly-Arg-pNA is a fluorogenic substrate for the measurement of protease activity. Gly-Arg-pNA undergoes hydrolysis and releases the fluorescent product p-nitroaniline. p-nitroaniline is in a fluorescent state under ultraviolet light irradiation and can emit a fluorescent signal .
|
-
- HY-P4417
-
|
Peptides
|
Others
|
Ac-IEPD-AMC is a fluorogenic substrate for the determination of protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal .
|
-
- HY-P4417A
-
|
Fluorescent Dye
|
Others
|
Ac-IEPD-AMC TFA is a fluorescent substrate used to measure protease activity. Ac-IEPD-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC fluoresces under UV light irradiation and can emit fluorescent signals .
|
-
- HY-P4323
-
|
Peptides
|
Others
|
Boc-Gln-Arg-Arg-AMC is a fluorogenic substrate for the determination of protease activity. Boc-Gln-Arg-Arg-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal .
|
-
- HY-P4408
-
|
Peptides
|
Others
|
Ac-Arg-Gly-Lys-AMC is a fluorogenic substrate for the determination of protease activity. Ac-Arg-Gly-Lys-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal .
|
-
- HY-P4416
-
|
Peptides
|
Others
|
Ac-Gly-Ala-Lys(Ac)-AMC is a fluorogenic substrate for the determination of protease activity. Ac-Gly-Ala-Lys(Ac)-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal .
|
-
- HY-P4419
-
|
Peptides
|
Others
|
Boc-Asp(OBzl)-Pro-Arg-AMC is a fluorogenic substrate for the determination of protease activity. Boc-Asp(OBzl)-Pro-Arg-AMC undergoes hydrolysis and releases the fluorescent product 7-amino-4-methylcoumarin (AMC). AMC is fluorescent under UV light and can emit a fluorescent signal .
|
-
- HY-P10142
-
|
Fluorescent Dye
|
Others
|
Ac-YEVD-AMC is a fluorescent substrate of caspase-1/11. Ac-YEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection when treating with cell lysate .
|
-
- HY-P2065
-
|
Fluorescent Dye
|
Others
|
Ac-VEID-AMC is a fluorescent substrate for the determination of caspase-6 and related cysteine protease activities (Ex=340-360 nm, Em=440-460 nm) .
|
-
- HY-P3791
-
|
Fluorescent Dye
|
Others
|
N-Succinyl-Ile-Ile-Trp-AMC is a peptide-based fluorescent substrate or probe for carboxypeptidase Y (CPY) .
|
-
- HY-P4400
-
|
Fluorescent Dye
|
Others
|
Z-VDVAD-AFC is a fluorogenic substrate. Z-VDVAD-AFC is used to measure the activity of cysteine protease 3 (Caspase-3). Z-VDVAD-AFC undergoes hydrolysis to release 7-amino-4-trifluoromethylcoumarin (AFC). AFC is fluorescent under ultraviolet light and can emit fluorescent signals .
|
-
- HY-P4406
-
|
Peptides
|
Others
|
Abz-AGLA-Nba is a fluorogenic substrate for the determination of protease activity. Abz-AGLA-Nba is hydrolyzed to release aminoacyl benzimide (Abz-AGLA) and 2-naphthylaminoacyl (Nba). The product Abz-AGLA produced by this hydrolysis reaction is fluorescent under ultraviolet light and can emit a fluorescent signal .
|
-
- HY-P10144
-
|
Fluorescent Dye
|
Others
|
Ac-Orn-Phe-Arg-AMC is a potent fluorescent substrate of cd–Tryptase and β 1-Tryptase. Ac-Orn-Phe-Arg-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection .
|
-
- HY-P2615
-
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Fluorescent Dye
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Cancer
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Ac-VDVAD-AFC is a caspase-specific fluorescent substrate. Ac-VDVAD-AFC can measure caspase-3-like activity and caspase-2 activity and can be used for the research of tumor and cancer .
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- HY-P10174
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- HY-P4425
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Peptides
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Others
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Gly-Phe-AFC is a fluorescent substrate, which is a peptide sequence composed of glycine and phenylalanine, linked to the fluorescent group AFC .
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- HY-P5990
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PSMA
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Cancer
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Prostate Specific Antigen Substrate is a prostate specific antigen (PSA) fluorescent substrate. Prostate Specific Antigen Substrate can be used for detect enzymatic activity of PSA .
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- HY-P4931
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Mca-Lys-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2
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MMP
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Cancer
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Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 (FS-6) is a fluorescent peptide that is a quenched MMP peptide substrate. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 can be used for real-time quantification of MMP enzymatic activity. Mca-Lys-Pro-Leu-Gly-Leu-Dap(Dnp)-Ala-Arg-NH2 is an elongated peptide of MMP substrate (FS-1) and is active against collagenases (MMP-1, MMP-8, MMP-13 ) and MT1-MMP with higher specificity constants than FS-1 . (Ex/Em=325 nm/400 nm)
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- HY-P4401
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Caspase
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Others
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Z-VEID-AFC is a fluorescent substrate for caspase-6 .
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- HY-P4343
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Peptides
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Others
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Lys(Abz)-Pro-Pro-pNA is a fluorescent peptide substrate of PepP .
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- HY-P4344
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MetAP
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Others
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Met-Gly-Pro-AMC is a fluorescent peptide substrate of MetAP2 .
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- HY-P1853
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- HY-P4443
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Peptides
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Others
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Ac-GAK-AMC is a fluorescent substrate that can be used to measure protease activity. AMC (7-amino-4-methylcoumarin) is a fluorescent group. In the presence of protease, the protease will hydrolyze Ac-GAK-AMC into a fluorescent product .
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- HY-P4364
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Peptides
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Others
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Suc-Phe-Leu-Phe-4MβNA is a fluorescent peptide substrate .
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- HY-137834A
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Peptides
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Others
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Gly-Pro-AMC is a biological active peptide. (This is a fluorescent dipeptidylaminopeptidase IV substrate, Abs/Em=353/442 nm.)
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- HY-P3722
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Fluorescent Dye
MMP
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Others
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Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE; ADAM17), ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis .
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- HY-P3722A
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Fluorescent Dye
MMP
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Others
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Mca-PLAQAV-Dpa-RSSSR-NH2 is a fluorescent peptide and as one of fluorescent substrates of TNF-α converting enzyme (TACE); ADAM17, ADAM 9 and ADAM 10. Mca-PLAQAV-Dpa-RSSSR-NH2 is a substrate based on fluorescence resonance energy transfer, and its activity can be determined by the change of fluorescence intensity during pyrolysis .
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- HY-P4428
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Peptides
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Others
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Z-Val-Lys-Met-AMC is a fluorescent substrate that can be used to detect the β-secretase activity of cathepsin B .
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- HY-P1883
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Fluorescent Dye
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Infection
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Bacterial Sortase Substrate III, Abz/DNP is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.
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- HY-P4429
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Peptides
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Others
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Z-YVAD-AFC is a fluorescent substrate for Caspase-1, which can be used to detect and measure the activity of Caspase-1 .
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- HY-P5355
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Peptides
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Others
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Z-Gly-Gly-Arg-AFC is a biological active peptide. (Plasminogen Activator Acrosine Substrate, fluorescent Z-Gly-Gly-Arg-AFC)
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- HY-P1883A
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Fluorescent Dye
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Infection
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Bacterial Sortase Substrate III, Abz/DNP TFA is an internally quenched fluorescent peptide substrate. Staphylococcus aureus transpeptidase sortase A (SrtA) reacts with its native substrate Bacterial Sortase Substrate III, Abz/DNP, cleaving it and catalyzing the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Cleavage of this substrate can be monitored at Ex/Em=320 nm/420 nm.
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- HY-P10073
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Fluorescent Dye
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Others
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Calpain-1 substrate, fluorogenic serves as a sensitive and specific substrate for calpain-1 that cleaves Tyr-Gly bond and results in enhanced fluorescence .(Ex/Em = 490 nm/518 nm)
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- HY-P2176
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Z-Leu-Arg-Gly-Gly-AMC
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Biochemical Assay Reagents
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Others
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Z-LRGG-AMC (Z-Leu-Arg-Gly-Gly-AMC) is a fluorescent substrate that hydrolyzes and releases free fluorescent 7-amino-4-methylcoumarin (AMC). Detection wavelength of AMC: Ex/Em=360/460 nm .
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- HY-P4545
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Peptides
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Others
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Abz-Gly-p-nitro-Phe-Pro-OH is the fluorescent substrate angiotensin I converting enzyme (ACE-I) with 355 nm excitation and 405 nm emission wavelengths .
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- HY-P0248F
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Peptides
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Others
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Kemptide, 5-FAM labeled is a biological active peptide. (Kemptide is a phosphate acceptor peptide that serves as a synthetic substrate for PKA (Km = 16 μM). The corresponding fluorescent and biotinylated peptides are also proven to be good substrates for PKA. 5-FAM labeled HY-P0248)
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- HY-D1532
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Parasite
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Others
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DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS is a fluorescent dye, and can be applied in a fluorogenic substrate for an aspartyl proteinase from human malaria parasite .
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- HY-W436471
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Peptides
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Others
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H-Ala-Pro-AFC is a biological active peptide. (This is a fluorescent peptide, Abs/Em=380/500nm. It is a substrate for dipeptidyl peptidase IV (DPP IV) and Xaa-Pro dipeptidase.)
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- HY-P4217
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Cathepsin
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Others
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Z-Gly-Pro-Arg-AMC hydrochloride a fluorescent trypsin and cathepsin K substrate. Z-Gly-Pro-Arg-AMC hydrochloride can be used to determine trypsin and cathepsin K activity .
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- HY-P4404
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Peptides
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Others
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Abz-GIVRAK(Dnp) is the most efficient substrate for cathepsin B and is highly selective for this enzyme among lysosomal cysteine proteases. After Abz-GIVRAK(Dnp) is hydrolyzed, aminoacylbenziminosulfosuccinic acid (Abz-SAS) is released, and dinitrobenzoyl (Dnp) is also released. The product of this hydrolysis reaction, Abz-SAS, is fluorescent under ultraviolet light and can emit a fluorescent signal .
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- HY-P5378
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Cathepsin S substrate
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Peptides
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Others
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Ac-KQKLR-AMC (Cathepsin S substrate) is a biological active peptide. (Cathepsins are a class of globular lysosomal proteases, playing a vital role in mammalian cellular turnover. They degrade polypeptides and are distinguished by their substrate specificities. Cathepsin S is a cysteine proteinase involved in the pathogenesis of autoimmune diseases, atherosclerosis, cancer, obesity and related diseases.This peptide is a cathepsin S substrate fluorescently labeled with AMC (Ex/Em=354 nm/442 nm). It can be used to measure cathepsin S activity.)
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- HY-P5356
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Plasmin substrate
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Peptides
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Others
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H-D-Val-Leu-Lys-AFC (Plasmin substrate) is a biological active peptide. (This is a fluorescent plasmin substrate, Abs/Em=380/500 nm.Plasmin belongs to the family of serine proteases. It plays a key role in fibrinolysis by dissolving fibrin in blood clots. Besides fibrinolysis, plasmin is also involved in such physiological and pathological processes as wound healing, liver repair, and the maintenance of liver homeostasis.)
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- HY-P3475
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Fluorescent Dye
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Others
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Mca-VDQMDGWK-(Dnp)-NH2 is a fluorogenic substrate for caspase-3 and can be used to quantify caspase-3 activity .
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- HY-P3749
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Fluorescent Dye
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Others
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Mca-(ala7,lys(dnp)9)-bradykinin is a sensitive fluorogenic substrate for ECE-1 (endothelin-converting enzyme-1). The incorporation of a (7-methoxycoumarin-4-yl)acetyl (Mca) fluorescent group and a 2,4-dinitrophenyl (Dnp) quenching group has resulted in a large fluorescence increase upon substrate cleavage .
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- HY-P3110
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Fluorescent Dye
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Others
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Ac-WLA-AMC is a fluorogenic substrate of caspase-3. Ac-WLA-AMC is cleaved to release the fluorescent moiety 7-amino-4-methylcoumarin (AMC), which can be used to quantify the β5c subunit activity .
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- HY-P10172
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- HY-P5481
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Peptides
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Others
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DABCYL-LPETG-EDANS is a biological active peptide. (This 5-amino acid peptide is a sortase substrate, C-terminal sorting signal. Sortase cleaves surface proteins at the LPXTG motif and catalyzes the formation of an amide bond between the carboxyl group of threonine and the amino group of cell-wall crossbridges. Sortases are a family of Gram-positive transpeptidases responsible for anchoring surface protein virulence factors to the peptidoglycan cell wall layer. Cleavage of this FRET substrate by sortase reveals the fluorescent signal, Abs/Em = 340/490 nm.)
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- HY-P1003
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Fluorescent Dye
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Others
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Ac-DEVD-AMC is a fluorescent substrate of caspase-3/caspase-7. When treating Ac-DEVD-AMC with cell lysate, Ac-DEVD-AMC releases amino-4-methylcoumarin (AMC) for fluorescence detection, with an excitation wavelength of 380 nm and an emission wavelength of 460 nm .
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- HY-P4593
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Thrombin
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Others
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Z-Gly-Pro-Arg-4MβNA is the cleavage of the substrate of thrombin to release free 4-methoxy-2-naphthylamine (4MβNA). Free 4MβNA can be captured by 5-nitrosalicylaldehyde to produce an insoluble yellow fluorescent and marks the site of thrombin activity .
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- HY-P4899
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Beta-secretase
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Neurological Disease
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RE (EDANS) EVNLDAEFK (DABCYL) R is an EDANS and DABCYL double-labeled peptide,serves as a fluorescent substrate for BACE1(Em=360nm,Ex=528nm). RE (EDANS) EVNLDAEFK (DABCYL) R can be used for BACE1 activity measurement and the enzyme activity level is directly proportional to the fluorescence reaction .
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- HY-P4920
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Fluorescent Dye
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Others
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Mca-SEVNLDAEFK(Dnp)-NH2 contains a highly fluorescent 7-methoxycoumarin group that is efficiently quenched by resonance energy transfer to the 2,4-dinitrophenyl group. It can be used to measure the activities of peptidases that are capable of cleaving an amide bond between the fluorescent group and the quencher group, causing an increase in fluorescence, such as can be used to measure the activity of BACE-1 .
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- HY-P4919
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Beta-secretase
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Others
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Mca-SEVNLDAEFK(Dnp) is a Beta-secretase 1 (BACE-1) peptide FRET substrate, containing the 'Swedish' Lys-Met/Asn-Leu mutation of the amyloid precursor protein (APP) β-secretase cleavage site. Cleavage at -Leu-Asp- of Mca-SEVNLDAEFK(Dnp) liberates the highly fluorescent 7-methoxycoumarin (Mca) fragment from the proximity quenching effect of the 2,4-dinitrophenyl (Dnp) internal quencher resulting in a large and easily detectable increase in fluorescence intensity.
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Cat. No. |
Product Name |
Category |
Target |
Chemical Structure |
Cat. No. |
Product Name |
Chemical Structure |
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- HY-W027544S
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d3 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3].
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- HY-W027544S1
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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Cat. No. |
Product Name |
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Classification |
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- HY-W027544S1
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5-(Dimethyl-d6-amino)-N-(prop-2-ynyl)naphthalene-1-sulfonamide
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Alkynes
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(7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is the deuterium labeled MCA[1]. MCA is a coumarin derivative. MCA quantitates platelet-activating factor by high-performance liquid chromatography with fluorescent detection. MCA can modify FRET peptide substrates for analyzing protease activities[2][3]. (7-Methoxy-2-oxo-2H-chromen-4-yl)-acetic acid-d6 is a click chemistry reagent, it contains an Alkyne group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing Azide groups.
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